多酚氧化酶 (菌菇) | ||||||||||||||
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參考價格 |
編號 | 含量 | 規(guī)格 | 包裝 | 價格 | 產(chǎn)地 | 備注 |
E1660 | >500U/mg | BR | 25ku | 590 | 國 | -20℃ |
概述
Polyphenol oxidase (tyrosinase) is a bifunctional, copper-containing oxidase having catecholase and cresolase activity. It is responsible for browning reactions through the phylogenetic scale. The enzyme has a molecular weight of 128 kDa. It is a tetramer containing four atoms of copper per molecule and two binding sites for aromatic compounds including phenolic substrates. There is a distinct binding site for oxygen. The optimum pH range is 6-7.
適宜溫度25℃。
最佳PH:6.0~7.0。
技術(shù)規(guī)格
來源:Mushrooms (Agaricus bisporus)
性狀:A lyophilized powder.
酶活:≥500 units per mg dry weight
酶活定義:One unit causes an increase in the absorbance at 280 nm of 0.001 per minute at 25°C, pH 6.5, using L-tyrosine as substrate.
儲存條件:-20°C.
用途:生化試劑
可催化含酚基化合物(如酪氨酸、酚類化合物等)氧化成醌的酶。此類酶廣泛存在于自然界,植物中分布很廣,如馬鈴薯、茶葉、漆樹乳汁中含量相當高。高等動物的皮膚、毛發(fā)、角膜中的黑色素也是由于此酶氧化酪氨酸所生成的產(chǎn)物。蘑菇等菌類及其他微生物中也產(chǎn)生此類酶。通常從野蘑菇中提取,系生物催化劑之一,含有銅離子的蛋白質(zhì)。等電點<5.0;含氮量約13.6%。含銅量約0.25%。紫外吸收峰在273nm和330nm處。
其它包裝規(guī)格請按需詢
多酚氧化酶,酪氨酸酶 (菌菇) Polyphenol oxidase